NAMPT Inhibitor Screening Assay Kit
The NAMPT Inhibitor Screening Assay Kit is a 96-well or 384-well fluorescent based assay designed to measure the activity of NAMPT (nicotinamide phosphoribosyltransferase) for screening and profiling applications. The kit contains enough purified NAMPT enzyme, NAMPT Assay Buffer, NAMPT Dilution Buffer, ATP, Nicotinamide, PRPP and ethanol for 100 or 400 enzyme reactions.
This product has been cited 1 time.
- Fluorescent microplate reader capable of reading λexc/λem = 340 nm/460 nm
- Adjustable micropipettor and sterile tips
96 reactions
Catalog # | Name | Amount | Storage |
91004 | Human NAMPT (PBEF1), GST-Tag* | 50 µg | -80°C |
NAMPT Dilution Buffer | 3 ml | -80°C | |
79946 | 4x NAMPT Assay Buffer | 2 x 750 µl | -80°C |
79686 | 400 µM ATP | 250 µl | -80°C |
400 µM Nicotinamide | 250 µl | -80°C | |
800 µM PRPP | 250 µl | -80°C | |
30% Ethanol | 1 ml | -80°C | |
79685 | Low binding black microtiter 96-well plate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
384 reactions
Catalog # | Name | Amount | Storage |
91004 | Human NAMPT (PBEF1), GST-Tag* | 60 µg | -80°C |
NAMPT Dilution Buffer | 3 ml | -80°C | |
79946 | 4x NAMPT Assay Buffer | 3 x 750 µl | -80°C |
79686 | 400 µM ATP | 2 x 250 µl | -80°C |
400 µM Nicotinamide | 2 x 250 µl | -80°C | |
800 µM PRPP | 2 x 250 µl | -80°C | |
30% Ethanol | 1 ml | -80°C | |
79961 | Low binding black microtiter 96-well plate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the formation of nicotinamide mononucleotide from nicotinamide and 5-phosphoribosyl-1-pyrophosphate (PRPP). It is the rate limiting enzyme in the mammalian NAD (nicotinamide adenine dinucleotide) biosynthesis pathway. NAMPT is thought to be involved in many important biological processes, including metabolism, stress response and aging. NAMPT is often overexpressed in cancer cells, an adaptation required to sustain the higher energetic need of the cells. In addition, it also plays roles in proliferation, invasion, stemness, angiogenesis, immune regulation, and drug resistance of tumors, making it an attractive target candidate for cancer therapy.
Ramsey K., et al., 2009, Science 324 (5927): 651-4.
Nakahata Y., et al., 2009, Science 324 (5927) : 654-7.
Gasparrini M., et al., 2022, Int J Biochem Cell Biol. 145 : 106189.