NAMPT Inhibitor Screening Assay Kit

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Catalog #
71276
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Description

The NAMPT Inhibitor Screening Assay Kit is a 96-well or 384-well fluorescent based assay designed to measure the activity of NAMPT (nicotinamide phosphoribosyltransferase) for screening and profiling applications. The kit contains enough purified NAMPT enzyme, NAMPT Assay Buffer, NAMPT Dilution Buffer, ATP, Nicotinamide, PRPP and ethanol for 100 or 400 enzyme reactions.  

This product has been cited 1 time.

Synonyms
Nicotinamide phosphoribosyltransferase, NAmPRTase; Nampt, Pre-B-cell colony-enhancing factor 1 (Pre-B cell-enhancing factor), Visfatin, PBEF, PBEF1
Product Info
Storage and Usage
Citations1
Assay Kit Format
Fluorogenic
Materials Required But Not Supplied
  • Fluorescent microplate reader capable of reading λexc/λem = 340 nm/460 nm
  • Adjustable micropipettor and sterile tips
Format

96 reactions

Catalog # Name Amount Storage
91004 Human NAMPT (PBEF1), GST-Tag* 50 µg -80°C
  NAMPT Dilution Buffer 3 ml -80°C
79946 4x NAMPT Assay Buffer 2 x 750 µl -80°C
79686 400 µM ATP 250 µl  -80°C
  400 µM Nicotinamide 250 µl  -80°C
  800 µM PRPP 250 µl  -80°C
  30% Ethanol 1 ml -80°C
79685 Low binding black microtiter 96-well plate 1 Room Temp

* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.


384 reactions

Catalog # Name Amount Storage
91004 Human NAMPT (PBEF1), GST-Tag* 60 µg -80°C
  NAMPT Dilution Buffer 3 ml -80°C
79946 4x NAMPT Assay Buffer 3 x 750 µl -80°C
79686 400 µM ATP 2 x 250 µl  -80°C
  400 µM Nicotinamide 2 x 250 µl  -80°C
  800 µM PRPP 2 x 250 µl  -80°C
  30% Ethanol 1 ml -80°C
79961 Low binding black microtiter 96-well plate 1 Room Temp

* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.

UniProt #
P43490
Background

Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the formation of nicotinamide mononucleotide from nicotinamide and 5-phosphoribosyl-1-pyrophosphate (PRPP). It is the rate limiting enzyme in the mammalian NAD (nicotinamide adenine dinucleotide) biosynthesis pathway.  NAMPT is thought to be involved in many important biological processes, including metabolism, stress response and aging. NAMPT is often overexpressed in cancer cells, an adaptation required to sustain the higher energetic need of the cells. In addition, it also plays roles in proliferation, invasion, stemness, angiogenesis, immune regulation, and drug resistance of tumors, making it an attractive target candidate for cancer therapy.

References

Ramsey K., et al., 2009, Science 324 (5927): 651-4.
Nakahata Y., et al., 2009, Science 324 (5927) : 654-7.
Gasparrini M., et al., 2022, Int J Biochem Cell Biol. 145 : 106189.