Kynureninase Inhibitor Screening Assay Kit

Catalog #
79514
$830 *
Size: 100 reactions
Qty
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Description

The Kynureninase (KYNU) Inhibitor Screening Assay Kit is designed to measure human KYNU enzyme inhibition. The KYNU Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are mixed and the reaction is initiated through addition of the substrate 3-hydroxy-DL-kynurenine (3-HK). After a room temperature incubation, activity is determined by measuring the fluorescence of the reaction product (ex. 315 nm, em. 415 nm).

Synonyms
activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Fluorogenic
Supplied As
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.
Format

Catalog #

Component

Amount

Storage

79485

KYNU His-tag*

2 µg

-80°C

Avoid freeze/ thaw cycles!

 

3X KYNU Assay Buffer

10 ml

-20°C

 

3-HK Substrate

400 µl

-80°C

79685

Black, low binding NUNC microtiter plate

1

Room Temp

UniProt #
Q16719
Background
IDO1/2 and TDO overexpression in tumor cells promotes tryptophan depletion in the microenvironment, resulting in suppression of the T-cell mediated immune response. IDO1/2 and TDO catalyze the breakdown of tryptophan into kynurenine, and subsequently KYNU cleaves kynurenine into anthranilic acid, a precursor of tryptophan. High levels of KYNU have been found in lymphoma, breast, liver, pancreas, and urothelial cancers, and is thought to promote tumor aggressiveness and metastasis. KYNU levels are also elevated in many chronic inflammatory skin diseases such as psoriasis and atopic dermatitis inflammatory, making KYNU attractive as a potential therapeutic and prognostic target.
References

1. Phillips, R.S. 2014. Arch Biochem Biophys. Feb 15; 544:69-74.

2. Badawy, AA. 2017. Int J Tryptophan Res. Mar 15;10: 1178646917691938.

3. Harden, J.L., et al. 2016. J Allergy Clin Immunol. Jun;137(6):1830-1840.