The Kynureninase (KYNU) Inhibitor Screening Assay Kit is designed to measure human KYNU enzyme inhibition. The KYNU Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are mixed and the reaction is initiated through addition of the substrate 3-hydroxy-DL-kynurenine (3-HK). After a room temperature incubation, activity is determined by measuring the fluorescence of the reaction product (ex. 315 nm, em. 415 nm).
IDO1/2 and TDO overexpression in tumor cells promotes tryptophan depletion in the microenvironment, resulting in suppression of the T-cell mediated immune response. IDO1/2 and TDO catalyze the breakdown of tryptophan into kynurenine, and subsequently KYNU cleaves kynurenine into anthranilic acid, a precursor of tryptophan. High levels of KYNU have been found in lymphoma, breast, liver, pancreas, and urothelial cancers, and is thought to promote tumor aggressiveness and metastasis. KYNU levels are also elevated in many chronic inflammatory skin diseases such as psoriasis and atopic dermatitis inflammatory, making KYNU attractive as a potential therapeutic and prognostic target.
References
1. Phillips, R.S. 2014. Arch Biochem Biophys. Feb 15; 544:69-74.
2. Badawy, AA. 2017. Int J Tryptophan Res. Mar 15;10: 1178646917691938.
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit for detailed protocol
Shipping Temperature
-80°C
Contraindications
DMSO > 1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.