IDH2(R140Q) Assay Kit

Catalog #
79309
$535 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The IDH2(R140Q) Assay Kit is designed to measure IDH2(R140Q) activity for screening and profiling applications by measuring NADPH consumption.

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This product has been cited 2 times.

Synonyms
Isocitrate Dehydrogenase 2, IDH2, Oxalosuccinate Decarboxylase, IDHM, IDH-2
Product Info
Storage and Usage
Citations2
Assay Kit Format
Fluorogenic
Supplied As
The IDH2(R140Q) Assay Kit comes in a convenient 96-well format, with enough purified recombinant IDH2(R140Q) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

NADPH (optional)*
Microplate reader capable of reading Fluorescence
Adjustable micropipettor and sterile tips


*Note: NADPH is not stable for multiple freeze/thaw cycles. We have provided the reagent as 2 x 100 μl to allow you to use only half the plate at a time. If you plan to use the same plate for additional experiments, please purchase additional NADPH and prepare a fresh 500 μM stock in H2O at the time of the assay.
Format
Catalog
Number

Component

Amount

Storage
71100 IDH2(R140Q) 3 µg -80°C


Avoid
freeze/
thaw

cycles!
  1x IDH2 Assay Buffer 20 ml -20°C
  α-Ketoglutarate (40 mM) 1 ml -20°C
  NADPH (500 μM) 2 x 100 μl -20°C
  NADPH Detection Reagent A 2 x 1 mg -20°C
  NADPH Detection Reagent B 2 x 1 mg -20°C
79685 96-well plate, black 1 Room Temp
UniProt #
P48735
Background
Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH2, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH2(R140Q) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH2(R140Q) mutation in various cancer types, including as glioma, chondrosarcoma, AML as well as small percentage of prostate, lung and colon cancers.
References

Wang, F. et. al. Science 340:622-626 (2013).