IDH1(R132H) Assay Kit
The IDH1(R132H) Assay Kit is designed to measure IDH1(R132H) activity for screening and profiling applications by measuring NADPH consumption.
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This product has been cited 1 time.
NADPH (optional)*
Microplate reader capable of reading Fluorescence
Adjustable micropipettor and sterile tips
*Note: NADPH is not stable for multiple freeze/thaw cycles. We have provided the
reagent as 2 x 100 μl to allow you to use only half the plate at a time. If you plan to use
the same plate for additional experiments, please purchase additional NADPH and
prepare a fresh 500 μM stock in H2O at the time of the assay.
Catalog Number |
Reagent |
Amount |
Storage |
|
71099 | IDH1(R132H) | 5 µg | -80°C | Avoid multiple freeze/thaw cycles! |
1x IDH1 Assay Buffer | 20 ml | -20°C | ||
α-Ketoglutarate (40 mM) | 1 ml | -20°C | ||
NADPH (500 μM) | 2 x 100 μl | -20°C | ||
NADPH Detection Reagent A | 2 x 1 mg | -20°C | ||
NADPH Detection Reagent B | 2 x 1 mg | -20°C | ||
79685 | 96-well plate, black | 2 | Room Temp |
Wang, F. et. al. Science 340:622-626 (2013).