IDH1(R132H) Assay Kit

Catalog #
79376
$535 *
Size: 96 reactions
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*US Pricing only. For international pricing, please contact your local distributor.
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Description

The IDH1(R132H) Assay Kit is designed to measure IDH1(R132H) activity for screening and profiling applications by measuring NADPH consumption.

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This product has been cited 1 time.

Synonyms
Isocitrate Dehydrogenase 1, IDH1, Oxalosuccinate Decarboxylase, IDHM, IDH-1
Product Info
Storage and Usage
Citations1
Assay Kit Format
Fluorogenic
Supplied As
The IDH1(R132H) Assay Kit comes in a convenient 96-well format, with enough purified recombinant IDH1(R132H) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

NADPH (optional)*
Microplate reader capable of reading Fluorescence
Adjustable micropipettor and sterile tips


*Note: NADPH is not stable for multiple freeze/thaw cycles. We have provided the
reagent as 2 x 100 μl to allow you to use only half the plate at a time. If you plan to use
the same plate for additional experiments, please purchase additional NADPH and
prepare a fresh 500 μM stock in H2O at the time of the assay.

Format
Catalog
Number

Reagent

Amount

Storage
71099 IDH1(R132H) 5 µg -80°C


Avoid multiple
freeze/thaw
cycles!
  1x IDH1 Assay Buffer 20 ml -20°C
  α-Ketoglutarate (40 mM) 1 ml -20°C
  NADPH (500 μM) 2 x 100 μl -20°C
  NADPH Detection Reagent A 2 x 1 mg -20°C
  NADPH Detection Reagent B 2 x 1 mg -20°C
79685 96-well plate, black 2 Room Temp
UniProt #
O75874
Background
Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH1, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH1(R132H) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH1(R132H) mutation in various cancer types, including glioma, chondrosarcoma, and AML as well as a small percentage of prostate, lung and colon cancers.
References

Wang, F. et. al. Science 340:622-626 (2013).