IDH1(R132H) Assay Kit

Catalog #: 79376
Size: 96 reactions
$495
Background:
Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH1, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH1(R132H) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH1(R132H) mutation in various cancer types, including glioma, chondrosarcoma, and AML as well as a small percentage of prostate, lung and colon cancers.
Description:
The IDH1(R132H) Assay Kit is designed to measure IDH1(R132H) activity for screening and profiling applications by measuring NADPH consumption.
Synonym(s):
Isocitrate Dehydrogenase 1, IDH1, Oxalosuccinate Decarboxylase, IDHM
Supplied As:
The IDH1(R132H) Assay Kit comes in a convenient 96-well format, with enough purified recombinant IDH1(R132H) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Useful for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Wang, F. et. al. Science 340:622-626 (2013).

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes
Product Type:
Assay Kit
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Product Datasheets

79376.pdf