COX1, FLAG-His-tags

Catalog #: 71110
Size: 100 µg
$455
Species: human
Host Species: Sf9 insect cells
MW: 70.5 kDa
Genbank #: NM_000962
Tag(s): C-terminal FLAG-His-tags
a.a: 1-599 (end)
Description:
Human COX1 protein, GenBank Accession No. NM_000962, a.a. 1-599 (end), with C-terminal FLAG-His-tag, MW=70.5 kDa, expressed in a baculovirus infected Sf9 cell expression system.
UniProt
P23219
Synonym(s):
Cox, COX1, COX 1, COX 2, COX2, COX1 Protein, Cyclooxygenase-1, Cox-1, Prostaglandin-Endo-peroxide Synthase 1, Prostaglandin G/H Synthase and Cyclooxygenase, PTGS1, PES-1, PGHS-1, Prostaglandin H2 Synthase 1, PTGHS, COX1
Assay Conditions:
Mixed various concentrations (0-200 nM) of enzyme in Assay buffer (100 mM Tris-HCl ph 8, 3 uM EDTA) with 5 uM Hematin for 5 min then added 100 uM Ampliflu Red and 50 uM arachidonic acid (Cayman Chemical # 90010.1 peroxide free) and measured fluorescence (ex 535 em 590) after 5 min. Reaction volume was 100 ul.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 100 µg/mL Flag peptide, 300 µM diethyldithiocarbamate, 0.1% Triton X-100 and 20% Glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at -80°C

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Scott, B.T., et al., Blood Coagul Fibrinolysis. 2002 Sep;13(6):519-31.
2. Funk, C.D., et al., FASEB J. 1991 Jun;5(9):2304-12.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes
Product Type:
Recombinant Protein
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Product Datasheets

71110 Lot 170227.pdf