Carbonic Anhydrase 2 (CA2), His-tag

Catalog #: 71094
Size: 100 µg
$465
Species: Human
Host Species: E. coli
MW: 30 kDa
Genbank #: NM_000067
Tag(s): C-terminal His-tag
a.a: 1–260(end)
Description:
Human carbonic anhydrase II, also known as CA2, (GenBank Accession No. NM_000067), full length (a.a. 1-260(end)) with C-terminal His-tag, MW = 30 kDa, expressed in an E. coli cell expression system.
UniProt
P00918
Synonym(s):
carbonic anhydrase II, CA2
Specific Activity:
1,400 pmol/min/μg
Assay Conditions:
15 mM Tris-HCl, pH 7.4, 100 mM NaCl, 3 mM p-Nitrophenyl acetate (PNPA) and 0-2 μg CA2. Incubate for 30 minutes at room temperature. Absorbance is measured at 405 nm.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 106 mM imidazole and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Loiselle, F.B., et al., Am J Physiol Cell Physiol. 2004 Jun;286(6):C1423-33
2. Shah, G.N., et al., Hum Mutat. 2004 Sep;24(3):272
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes-Anhydrase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

71094_Lot130509.pdf