Carbonic Anhydrase 1 (CA1), His-tag

Catalog #: 71093
Size: 100 µg
$465
Species: Human
Host Species: E. coli
MW: 29.7 kDa
Genbank #: NM_001128829
Tag(s): C-terminal His-tag
a.a: 1–261(end)
Description:
Human carbonic anhydrase I, also known as CA1, (GenBank Accession No. NM_001128829), full length (a.a. 1-261(end)) with C-terminal His-tag, MW = 29.7 kDa, expressed in an E. coli cell expression system.
UniProt
P00915
Synonym(s):
carbonic anhydrase I, CA1
Specific Activity:
2,000 pmol/min/μg
Assay Conditions:
15 mM Tris-HCl, pH 7.4, 100 mM NaCl, 3 mM p-Nitrophenyl acetate (PNPA) and 0-2 μg CA1. Incubate for 30 minutes at room temperature. Absorbance is measured at 405 nm.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 185 mM imidazole and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Lowe, N., et al., Gene 1990 Sep 14;93(2):277-83.
2. Jover, T., et al., J Neurosci. 2002 Mar 15;22(6):2115-24.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes-Anhydrase
Product Type:
Recombinant Protein
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