TDO Inhibitor Screening Assay Kit
Catalog #
72023
$745
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Description
The TDO Inhibitor Screening Assay Kit is designed to measure TDO enzyme inhibition. The TDO Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation activity is measured by measuring the absorption of reaction product at λ = 320-325 nm.
This product has been cited 1 time.
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Synonyms
TDO2, tryptophan 2,3 dioxygenase, activity assay kit
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Product Data Gallery
Product Info
Storage and Usage
Citations1
Assay Kit Format
Colorimetric - UV Spectrophotometry
Supplied As
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.
Materials Required But Not Supplied
Spectrophotometer capable of measuring absorbance at λ = 320-325 nm.
Adjustable micropipettor and sterile tips
Format
Catalog Number |
Component |
Amount |
Storage |
|
71195 | TDO, His-Tag* | 50 µg | -80°C | Avoid freeze/ thaw cycles! |
73005 | TDO Reaction Solution | 2 x 10 ml | -80°C | |
73006 | TDO Assay Buffer | 1 ml | -80°C | |
79965 | UV transparent 96-well plate | 1 | Room Temp. |
*The concentration of TDO is lot-specific and will be indicated on the tube containing the enzyme.
UniProt #
P48775
Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein
synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as
a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an
immunosuppressive microenvironment by starving immune cells of L-Trp and releasing
degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3
dioxygenase (TDO) is upregulated in many tumors, providing cancer cells with an avenue for
immune evasion.
References
Li, J.S., et al. Arch Insect Biochem Physiol. 2007 Feb; 64(2): 74–87