NAMPT Inhibitor Screening Assay Kit

Catalog #: 79307
Size: 384 reactions
$1,045
Background:
NAMPT catalyzes the formation of nicotinamide mononucleotide from nicotinamide and 5-phosphoribosyl-1-pyrophosphate (PRPP). It is the rate limiting component in the mammalian NAD biosynthesis pathway. The protein is thought to be involved in many important biological processes, including metabolism, stress response and aging.
Description:
The NAMPT Inhibitor Screening Assay Kit is designed to measure NAMPT activity for screening and profiling applications.
Synonym(s):
Nicotinamide Phosphoribosyltransferase Protein, NAMPT, PBEF1, Visfatin, Pre-B-Cell Colony-Enhancing Factor 1, NMPT
Supplied As:
The NAMPT assay kit comes in a convenient 384-well format, with purified recombinant NAMPT enzyme, NAMPT assay buffer, NAMPT dilution buffer, ATP, Nicotinamide, PRPP, and Ethanol enough for 384 enzyme reactions.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Ramsey, K.M., et al. (2009) Science 324: 651-654.

Nakahata, Y., et al. (2009) Science 324: 654-657.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes
Product Type:
Assay Kit
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Product Datasheets

79307.pdf