Mouse IDO1 Inhibitor Screening Assay Kit

Catalog #
72041
$745 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The Mouse IDO1 Inhibitor Screening Assay Kit is designed to measure inhibition of the murine IDO1 enzyme. This kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at l=320-325 nm.

Synonyms
Indoleamine 2,3-dioxygenase 1, IDO, IDO1, activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Colorimetric - UV Spectrophotometry
Supplied As
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.
Format
Catalog # Component Amount Storage
71196 Mouse IDO1 His-Tag 40 µg -80°C Avoid freeze/ thaw cycles!
73001 IDO Reaction Solution 2 x 10 ml -80°C
73002 IDO1 Assay Buffer 1 ml -80°C
79965 UV transparent 96-well plate  1 Room Temp  
Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
References

1. Koblish, H.K., et al., Mol Cancer Ther, 2010; 9(2): 489-98
2. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
3. Seegers, N., et al. J Biomol Screen. 2014; 19(9):1266-74.