IDH1(R132H) Assay Kit

Catalog #: 79376
Size: 96 reactions
Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH1, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH1(R132H) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH1(R132H) mutation in various cancer types, including glioma, chondrosarcoma, and AML as well as a small percentage of prostate, lung and colon cancers.
The IDH1(R132H) Assay Kit is designed to measure IDH1(R132H) activity for screening and profiling applications by measuring NADPH consumption.
Isocitrate Dehydrogenase 1, IDH1, Oxalosuccinate Decarboxylase, IDHM
Supplied As:
The IDH1(R132H) Assay Kit comes in a convenient 96-well format, with enough purified recombinant IDH1(R132H) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions.

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Useful for screening small molecular inhibitors for drug discovery and HTS applications.

Wang, F. et. al. Science 340:622-626 (2013).

Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes
Product Type:
Assay Kit
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