GLS1 Inhibitor Screening Assay Kit

Catalog #

79596

$840 *

Size: 96 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The GLS1 Inhibitor Screening Assay Kit is designed to measure the hydrolase activity of GLS1 for screening and profiling applications.

Need us to run inhibitor screens or profile your compounds against GLS1? Check out our Metabolic Enzyme Screening Services.

This product has been cited 2 times.

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Synonyms

glutaminase, glutaminase transcript variant 1, GLS1, GAC, GAM, KGA, GLS., activity assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations2

Assay Kit Format

Fluorogenic

Supplied As

The GLS1 assay kit comes in a convenient 96-well format, with purified GLS1, its substrates, the Coupling reagent, and GLS1 buffer for 100 enzyme reactions. In addition, the kit includes the GLS1 inhibitor CB-839 for use as a control inhibitor.

Materials Required But Not Supplied

0.5 M DTT in aqueous solution
Adjustable micropipettor and sterile tips
Fluorescent microplate reader capable of reading ⅄ excitation = 340 nm; ⅄ emission = 460 nm

Format

Catalog Number	Component	Amount	Storage
71102	GLS1, His-tag **	1 µg	-80 °C	Avoid freeze/ thaw cycles!
	L-Glutamine (100 mM)	100 µl	-20 °C
	NAD+ (20mM)	1 ml	-20 °C
	Coupling reagent	10 µl	-20 °C
79948	4X GLS assay buffer*	2.5 ml	-20 °C
	CB-839 (100 µM)	20 µl	-20 °C
79685	96-well black microplate	1	Room Temp.

** The concentration of GLS1 is lot-specific and will be indicated on the tube containing the enzyme.

* Add 20 µl of 0.5 M DTT to 2.5 ml 4X GLS1 assay buffer before use.

UniProt #

O94925

Background

Kidney-type glutaminase (GLS1) is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of L-glutamine to L-glutamate and ammonia. GLS1 is primarily expressed in the brain and kidney where it catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. It plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney.

References

1. Mates, J. M., et al. (2013). Glutaminase isoenzymes as key regulators in metabolic and oxidative stress against cancer. Current Molecular Medicine 13(4): 514-534.
2. Gross, Matthew I., et al. (2014). Antitumor activity of the glutaminase inhibitor CB-839 in triple-negative breast cancer. Molecular Cancer Therapeutics 13(4): 890-901.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Metabolic

Regulatory Status

Research Use Only