ALOX15, FLAG-tag

Catalog #: 50220
Size: 20 µg
$455
Species: Human
Host Species: Sf9 insect cells
MW: 75 kDa
Genbank #: NM_001140
Tag(s): N-terminal FLAG-tag
a.a: 2– 662(end)
Description:
ALOX-15, also known as 15LOX-1, omega-6 lipoxygenase, or arachidonate 15-lipoxygenase (GenBank Accession No. NM_001140), a.a. 2- 662(end), with N-terminal FLAG-tag, MW=75 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
UniProt
P16050
Synonym(s):
15LOX-1, omega-6 lipoxygenase, arachidonate 15-lipoxygenase
Assay Conditions:
ALOX activity was measured using a DPPP (diphenyl-1-Pyrenylphosphine) coupled assay. The enzyme (5.6 ng/µl) was incubated with 200 µM linoleic acid in DPBS buffer (pH 7.4) for 10 min at room temperature followed by addition of 100 µl of 0.125 mM DPPP in methanol. After 30 min incubation at room temperature, fluorescence was read at Ex350nm/Em380nm.
Formulation:
50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, and 100 ng/µl FLAG peptide.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Jiang, W.G., et al. (2006). Prostaglandins Leukot Essent Fatty Acids. 74 (4): 235-45.
2. Sigal, E., et al. (1988). Biochem. Biophys. Res. Commun. 157 (2): 457-64.
3. Bhattacharya S, et al. (2009). Tumour Biol. 30(4):185-99.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes-Oxygenase
Product Type:
Recombinant Protein
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Product Datasheets

50220_Lot110602.pdf