ACC2, His-tag, Strep-tag

Catalog #: 50203
Size: 10 µg
$475
Species: Human
Host Species: Sf9 insect cells
MW: 279 kDa
Genbank #: NM_001093
Tag(s): N-terminal His-tag and C-terminal Strep-tag
a.a: Full-length
Description:
Human ACC2 (GenBank Accession No. NM_001093), full length, with an Nterminal His-tag and C-terminal Strep-tag MW=279 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
O00763
Synonym(s):
acetyl-coa carboxylase acetyl coa acetyl coenzyme a ACC2, acetyl-Coenzyme A carboxylase alpha transcript variant 2, ACC-2, ACC 2, ACC -2
Specific Activity:
≥40 pmoles/min/µg
Assay Conditions:
A 25 μl ACC2 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM Sodium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO3 and 20 μM acetyl-CoA at 30°C for 55 min. ATP reduction is detected using ADP-Glo® Luminescent Assay Platform (Promega).
Formulation:
80 mM Tris-HCl, pH 8.0, 120 mM NaCl, 1 mM EDTA, 2 mM desthiobiotin, 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Locke G.A. et al., Arch. Biochem. Biophys. 475 (1), 72-79 (2008).
2. Abu-Elheiga L. et al., Proc. Natl. Acad. Sci. U.S.A. 97 (4), 1444-1449 (2000).

Application Reference(s):
A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)

Warning(s):
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Metabolic Enzymes
Product Type:
Recombinant Protein
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Product Datasheets

50203_Lot160415.pdf