ACC1, FLAG-His-tags

Catalog #: 50202
Size: 10 µg
$475
Species: Human
Host Species: Sf9 insect cells
MW: 271 kDa
Genbank #: NM_198834
Tag(s): C-terminal FLAG-His-tags
a.a: 1–2383(end)
Description:
Human ACC1 (acetyl-Coenzyme A carboxylase alpha transcript variant 1) (GenBank Accession No. NM_198834), a.a. 1–2383(end) with C-terminal FLAG and His-tags, MW = 270 kDa, expressed in Baculovirus infected Sf9 cell expression system.
UniProt
Q13085
Synonym(s):
acetyl-Coenzyme A carboxylase alpha transcript variant 1, ACC-1, ACC 1, ACC -1 acetyl-coa carboxylase acetyl coa acetyl coenzyme a
Specific Activity:
≥58 pmol/min/µg.
Assay Conditions:
A 25 μl ACC1 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected using ADP-Glo® Luminescent Assay Platform (Promega).
Formulation:
50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 10% glycerol, 1 mM DTT, 100 µg/ml FLAG peptide
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Sinilnikova, O.M. et al., Cancer Epidemiol. Biomarkers Prev. 16 (3), 409-415 (2007)
2. Olsen, J.V. et al., Cell 127 (3), 635-648 (2006)

Application Reference(s):
A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes-Carboxylase
Product Type:
Recombinant Protein
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Product Datasheets

50202_Lot_170208.pdf