MEG-2 (PTPN9), GST-tag

Catalog #: 30020
Size: 20 µg
$375
Species: Human
Host Species: E. coli
MW: 60.3 kDa
Genbank #: M83738
Tag(s): N-terminal GST-tag
a.a: 285–582
Background:
This PTP contains an N-terminal domain that shares a significant similarity with yeast SEC14, which is a protein that has phosphatidylinositol transfer activity and is required for protein secretion through the Golgi complex in yeast. This PTP was found to be activated by polyphosphoinositide, and is thought to be involved in signaling events regulating phagocytosis.
Description:
Protein tyrosine phosphatase (GenBank accession number M83738), a.a. 285-582 (catalytic domain) with N-terminal GST-tag, MW=60.3 kDa, expressed in an E. coli expression system.
Synonym(s):
MEG2, MEG-2, PTPN9
Specific Activity:
1070 pmole/min/µg
Assay Conditions:
20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 2 ng/µl MEG-2. Assay was done at RT for 15 min, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Formulation:
25 mM Tris-HCl, pH 8.0, 67 mM NaCl, 1.4 mM KCl, 50% glycerol, 10 mM glutathione, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s):
1. Q. Ying, et al., J. Cell Biochem. 2002, 86: 79.
2. H. Huynh, et al. J. Immunol. 2003, 171: 6661.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Non-rec.
Product Type:
Recombinant Protein
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Product Datasheets

30020_MEG2_lot_070725.pdf