MEG-1 (PTPN4), GST-tag Recombinant

Catalog #

30015

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Recombinant human MEG-1 (also known as PTPN4, protein tyrosine phosphatase non-receptor type 4), encompassing amino acids 637-926 (end). The construct contains an N-terminal GST-tag. The recombinant protein was affinity purified.

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Synonyms

Tyrosine-protein phosphatase non-receptor type 4, Protein-tyrosine phosphatase MEG1, MEG, PTPase-MEG1, PTPN4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

MEG-1 (GST-637-926(end))

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

60 kDa

Amino Acids

637-926(end)

Genbank #

NM_002830

UniProt #

P29074

Tag(s)

N-terminal GST-tag

Background

PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP acts at junctions between the membrane and the cytoskeleton and has been shown to interact with glutamate receptor delta 2 and epsilon subunits. It also is thought to play a role in signalling downstream of the glutamate receptors through tyrosine dephosphorylation.

References

1. K. Hironaka, et al., J. Biol.Chem. 2000, 275: 16167-16173
2. M. Gu, et al. Proc. Natl. Acad. Sci. 1996, 93: 12980-12985

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 10 µM DIFMUP. Incubated for 5 minutes at room temperature with various amounts of MEG-1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Non-rec.

Regulatory Status

Research Use Only