MEG-1 (PTPN4), GST-tag

Catalog #: 30015
Size: 20 µg
$375
Species: Human
Host Species: E. coli
MW: 60.3 kDa
Genbank #: NM_002830
Tag(s): N-terminal GST-tag
a.a: 637–926
Background:
PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP acts at junctions between the membrane and the cytoskeleton and has been shown to interact with glutamate receptor delta 2 and epsilon subunits. It also is thought to play a role in signalling downstream of the glutamate receptors through tyrosine dephosphorylation.
Description:
MEG-1 protein tyrosine phosphatase (PTP-MEG-1), Genbank Accession No. NM_002830, a.a. 637-926 (catalytic domain) with N-terminal GST-tag, MW=60.3 kDa, expressed in an E. coli expression system.
Synonym(s):
MEG1, MEG-1, PTPN4, Megakaryocyte Phosphatase
Specific Activity:
4.6 U/µg
Unit Definition:
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Assay Conditions:
The specific activity was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme for 5 minutes at 30°C.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s):
1. K. Hironaka, et al., J. Biol.Chem. 2000, 275: 16167-16173
2. M. Gu, et al. Proc. Natl. Acad. Sci. 1996, 93: 12980-12985
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Non-rec.
Product Type:
Recombinant Protein
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