MCL-1 TR-FRET Assay Kit

Catalog #
79506
$870 *
Size: 384 reactions
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Description

The MCL-1 TR-FRET Assay Kit is designed to measure the inhibition of MCL-1 binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, MCL-1, peptide ligand, and an inhibitor is incubated for 3 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against MCL-1? Check out our Apoptosis Screening Services or DNA Replication and Repair Screening Services.​ 

This product has been cited 3 times.

Synonyms
mcl1
Product Info
Storage and Usage
Citations3
Assay Kit Format
TR-FRET
Materials Required But Not Supplied
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
Format
Catalog # Component Amount Storage
40742 MCL-1 10 µg -80°C Avoid
freeze/
thaw
cycles!
79507 MCL-1 Peptide Ligand 400 rxns -80°C
30017 Anti-His Tb-labeled donor 10 µl -20°C
  Dye-labeled acceptor 10 µl -20°C
  3x MCL TR-FRET Assay Buffer 4 ml -20°C
79969 White, nonbinding, low volume, microtiter plate 1 Room Temp.
UniProt #
Q07820
References

1. Abid, M., et al. Curr Med Chem 2017; 24:4488