LSD1 (KDM1A) Homogeneous Assay Kit

Catalog #: 50108
Size: 384 reactions
LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.
The LSD1 Homogeneous Assay Kit is designed to measure activity of lysine-specific demethylase (LSD1) for screening and profiling applications. The key to the LSD1 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase detection. First, a sample containing LSD1 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
KDM1A, AOF2, LSD1, BHC110, LSD, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen

Assay Kit Components

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
1. Forneris F, et al. J Biol Chem 2006; 281(46):35289-95.
2. Zhou M, et al. Anal Biochem 1997; 253(2):162-8.
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Avoid freeze/thaw cycles
Scientific Category:
Product Type:
Assay Kit
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