LSD1 (KDM1A) Homogeneous Assay Kit

Catalog #: 50108
Size: 384 reactions
$540
Background:
LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.
Description:
The LSD1 Homogeneous Assay Kit is designed to measure activity of lysine-specific demethylase (LSD1) for screening and profiling applications. The key to the LSD1 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase detection. First, a sample containing LSD1 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
UniProt
O60341
Synonym(s):
KDM1A, AOF2, LSD1, BHC110, LSD, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Format:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
1. Forneris F, et al. J Biol Chem 2006; 281(46):35289-95.
2. Zhou M, et al. Anal Biochem 1997; 253(2):162-8.
Notes:
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Demethylase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50108.pdf