LSD1 (KDM1A) Chemiluminescent Assay Kit

Catalog #: 50109
Size: 96 reactions
$450
Background:
LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.
Description:
The LSD1 Chemiluminescent Assay Kit is designed to directly measure activity of human lysine-specific demethylase (LSD1) for screening and profiling applications. The key to the LSD1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing LSD1 enzyme is incubated with a sample containing assay buffer. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
UniProt
O60341
Synonym(s):
KDM1A, AOF2, LSD1, BHC110, LSD
Supplied As:
Kit comes in a convenient format, with a 96-well plate precoated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified LSD1 for 100 reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Demethylase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50109.pdf