LSD1 (KDM1A) Chemiluminescent Assay Kit

Catalog #

50109

$695 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The LSD1 Chemiluminescent Assay Kit is designed to directly measure activity of human lysine-specific demethylase (LSD1) for screening and profiling applications. The key to the LSD1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing LSD1 enzyme is incubated with a sample containing assay buffer. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

This product has been cited 5 times.

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Synonyms

KDM1A, AOF2, LSD1, BHC110, LSD

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Product Data Gallery

Product Info

Storage and Usage

Citations5

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with a 96-well plate precoated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified LSD1 for 100 reactions.

Materials Required But Not Supplied

DTT (Dithiothreitol), 0.5M (Sigma, Cat. # D0632)
TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog Number	Component	Amount	Storage
50100	LSD1	10 µg	-80°C	*Avoid* *freeze/ thaw* *cycles!*
52140J	Primary antibody 10	12.5 µl	-80°C
52130H	Secondary HRP-labeled antibody 1	10 µl	-80°C
	3x LSD1 assay buffer 2 (add DTT before use*)	4 ml	-20°C
52100	Blocking buffer 4	50 ml	+4°C
	HRP chemiluminescent substrate A (transparent bottle)	6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	6 ml	+4°C
	Microplate precoated with histone substrate	1	+4°C

*Adding DTT can affect potency of some inhibitors

UniProt #

O60341

Background

LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.

References

1. Forneris, F., et al., J Biol Chem. 2006; 281(46):35289-95.
2. Zhou, M., et al., Anal Biochem. 1997; 253(2):162-8.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Demethylase

Regulatory Status

Research Use Only