LSD1 Variant 1, GST-tag

Catalog #: 50204
Size: 50 µg
$450
Species: Human
Host Species: E. coli
MW: 104 kDa
Genbank #: NM_015013
Tag(s): N-terminal GST-tag
a.a: 158–end
Description:
Variant 2 Human LSD1 [also known as KDM1A (lysine (K)-specific demethylase 1A) or AOF2, amine oxidase (flavin containing) domain 2)] GenBank Accession No. NM_015013, a.a. 158-end with N-terminal GST-tag, MW=103 kDa, expressed in an E. coli expression system.
UniProt
O60341
Synonym(s):
KDM1A, AOF2, lysine (K)-specific demethylase 1A, amine oxidase (flavin containing) domain 2, LSD
Specific Activity:
≥0.50 pmole/min/μg
Assay Conditions:
10 μl reaction mix containing assay buffer with 50 mM Tris HCl pH 8.8, 50 mM NaCl, 1mM DTT, 0.01% Tween-20, histone H3 peptide substrate, and LSD1 (6.7 – 40 ng) add to the wells. Incubate for 1 hr. Add antibody against unmethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Formulation:
100 mm Na2HPO4 /NaH2PO4, pH 7.4
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes:

LSD1,GST-tag (BPS Cat. #50204) is optimized for use with BPS Bioscience's LSD1 Fluorescent Assay Kits including BPS Cat #50106 and BPS Cat #50107. 

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50204 Lot 170703-1.pdf