LIGHT:HVEM [Biotinylated] Inhibitor Screening Assay Kit

Catalog #

79684

$1,040 *

Size: 96 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The LIGHT:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LIGHT is coated on a 96-well plate. Next, HVEM is incubated with LIGHT on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against LIGHT:HVEM [Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

TNFSF14, CD258, HVEML, LIGHT, LTg, TR2, TNLG1D, tumor necrosis factor superfamily member 14, TNF superfamily member 14

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format

Catalog Number	Component	Amount	Storage
71266	LIGHT, His-Tag (Human)	10 µg	-80 °C	Avoid multiple freeze/thaw cycles!
71143	HVEM, Fc fusion, Biotin-labeled (Human)	5 µg	-80 °C
79311	3x Immuno Buffer 1	50 ml	-20 °C
79728	Blocking Buffer 2	50 ml	+4 °C
	Streptavidin-HRP	15 µl	-20 °C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp
79699	96-well white microplate	1	+4 °C

UniProt #

LIGHT: O43557; HVEM: Q92956

Background

Herpesvirus entry mediator (HVEM, also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14) or CD270) and LIGHT (tumor necrosis factor superfamily member 14 (TNFSF14) or CD258) mediate costimulatory responses in T cell activation and proliferation during the immune response. The HVEM/LIGHT co-signaling pathway plays important roles in several biological processes, including autoimmunity, inflammation, bacterial and virus infection, transplant rejection and cancer.

References

Granger, S.W., et al. Cytokine Growth Factor Rev. 2003, 14(3-4): 289-296
Del Rio, M.L., et al. J. Leukoc. Biol. 2010, 87(2): 223-235

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only