LDLR, FLAG-Tag, Eu-labeled Recombinant

Catalog #

79079

$455 *

Size: 50 µg

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Description

Recombinant human LDLR (low density lipoprotein receptor), encompassing amino acids 22-788. This construct contains a C-terminal FLAG-tag. The protein was affinity purified and labeled with trFluor™ Eu.

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Synonyms

low density lipoprotein receptor FH, FHC, LDLCQ2, LDL receptor, FH, FHC, FHCL1, LDLCQ2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PCSK9 [Biotinylated]: LDLR Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

LDLR (22-788-FLAG)-(trFluor-Eu)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol

86 kDa

Amino Acids

22-788

Genbank #

NM_000527

UniProt #

P01130

Tag(s)

C-terminal FLAG-Tag

Label

trFluor™ Eu (Ex/Em = 346nm / 617 nm) is a fluorophore with large Stokes shifts and an extremely long emission half-live. It is ideal for applications that require high sensitivity and for time-resolved fluorometry because a delay between excitation and the detection of emission minimizes interference. EU-labeled probes are characterized by a relatively high stability and are often insensitive to quenching. trFluor™ Eu is covalently and randomly conjugated to the target protein.

References

1. Holla, L., et al., BMC Cell Biol. 2007 Mar 1;8:9.
2. Qian, YW., et al., J Lipid Res. 2007 Jul;48(7):1488-98.

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was performed according to the PCSK9-LDLR TR-FRET Assay Kit (BPS Bioscience #72010) with PCSK9 [Biotinylated] enzyme titrated from 160 ng/rxn – 0.15 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cholesterol

Regulatory Status

Research Use Only

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Datasheets/Protocols

79079 Lot 210210-GB1

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LDLR, FLAG-Tag, Eu-labeled Recombinant

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