LDLR, FLAG-Avi-Tag (Mouse) Recombinant

Catalog #

71222

$455 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Mouse low density lipoprotein receptor (LDLR), also known as FH, FHC, and LDLCQ2, GenBank Accession No. NM_010700, a.a. 22-790, with C-terminal Flag-tag, MW=87 kDa87, expressed in a HEK293 cell expression system. *Note: LDLR is heavily glycosylated, resulting in higher molecular weight.

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Synonyms

FH, FHC, LDLR, LDLCQ2, low density lipoprotein receptor

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

HEK293 cells

Purity

≥88%

Formulation

40 mM Tris HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

87 kDa

Amino Acids

22-790 (end)

Genbank #

NM_010700

Tag(s)

C-terminal FLAG-Avi-tag

Background

The low density lipoprotein receptor (LDLR protein) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. In case of HIV-1 infection, it functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the LDLR protein, inducing LDLR degradation.

References

1. Holla, L., et al., BMC Cell Biol. 2007 Mar 1,8:9.
2. Qian, YW., et al., J Lipid Res. 2007 Jul,48(7):1488-98.
3. Fasano, T., et al., Athersclerosis. 2009 Mar,203(1):166-71.

Storage/Stability

At least 6 months at –80°C. Avoid freeze thaw cycles. Storing diluted protein is not recommended, if necessary, use a carrier protein (BSA 0.1 – 0.5%).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and screening small molecules for drug discovery.

Shipping Temperature

-80°C

Notes

* LDLR is heavily glycosylated, resulting in higher molecular weight. The two bands shown correspond to differing states of glycosylation.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cholesterol Related

Regulatory Status

Research Use Only

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Datasheets/Protocols

71222 lot 130110-D.pdf

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LDLR, FLAG-Avi-Tag (Mouse) Recombinant

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