HDAC3 Kinetic Assay Kit

Catalog #

53003

$550 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Kinetic HDAC3 Assay Kit is a complete assay system designed to measure histone deacetylase 3 (HDAC3) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC3 activity measurements. In addition, the kit includes purified HDAC3/NCOR2 enzyme complex and a potent HDAC inhibitor, SAHA, for use as a positive control. The Kinetic HDAC3 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC3 reaction on a microtiter plate is needed and then the HDAC3 activity level can be measured kinetically using a fluorescence reader.

This product has been cited 4 times.

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Synonyms

histone deacetylase, HDAC3, HDAC-3

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Product Info

Storage and Usage

Citations4

Assay Kit Format

Fluorogenic

Format

Assay Kit Components

UniProt #

O15379

References

1. Santo, L., et al., Blood. 2012 Mar 15;119(11):2579-89.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors of HDAC3 for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only

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HDAC3 Kinetic Assay Kit

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