The Kinetic HDAC2 Assay Kit is a complete assay system designed to
measure histone deacetylase 2 (HDAC2) activity kinetically for screening and profiling
applications. It comes in a convenient 96-well format, with all the reagents necessary for
100 fluorescent HDAC2 activity measurements. In addition, the kit includes purified
HDAC2 enzyme and a potent HDAC inhibitor, SAHA, for use as a positive and negative
control. The Kinetic HDAC2 Assay Kit is based on a unique fluorogenic substrate and
developer combination. This assay method eliminates dealing with the radioactivity,
extraction, and chromatography aspects of traditional assays. Using this kit, only one
simple step for setting up the HDAC2 reaction on a microtiter plate is needed and then
the HDAC2 activity level can be measured kinetically using a fluorescence reader.
1. Santo, L., et al., Blood. 2012 Mar 15;119(11):2579-89.
Storage/Stability
6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol.
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors of HDAC2 for drug discovery and HTS applications.
Shipping Temperature
-80°C
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.