HDAC1 Kinetic Assay Kit
The Kinetic HDAC1 Assay Kit is a complete assay system designed to measure histone deacetylase 1 (HDAC1) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified HDAC1 enzyme and a potent HDAC inhibitor, SAHA, for use as a positive and negative control. The Kinetic HDAC1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC1 reaction on a microtiter plate is needed and then the HDAC1 activity level can be measured kinetically using a fluorescence reader.
This product has been cited 10 times.
Fluorescent microplate reader
| Catalog # | Reagent | Amount | Storage | |
| 50051 | HDAC1 human recombinant enzyme | 10 µg | -80°C | Avoid Freeze/Thaw cycles |
| 50037 | Fluorogenic HDAC substrate 3 (5 mM) | 50 µl | -80°C | |
| 53029 | 10x HDAC Developer | 1 ml | -80°C | |
| Developer Dilution Buffer | 10 ml | -20°C | ||
| SAHA (1 mM) | 20 µl | -20°C | ||
| 50031 | HDAC Assay Buffer | 20 ml | -20°C | |
| BSA (1mg/ml) | 1 ml | -20°C | ||
| 79685 | black, low binding NUNC black microtiter plate |
1 | Room Temp | |
1. Santo, L., et al., Blood. 2012 Mar 15;119(11):2579-89.
2. Bradner, J.E., et al., Nat Chem Biol. 2010 Mar;6(3): 238-243.
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