PI3 Kinase [p110α (N345K)/p85α], His-Tag

Catalog #: 40646
Size: 10 µg
$370
Species: Human
Host Species: Sf9 insect cells
MW: p110α (N345K): 111 kDa, p85α: 86 kDa
Genbank #: p110α (N345K): NM_006218.2, p85α: NM_181523
Tag(s): p110α: N-terminal His-Tag, p85α: No Tag
a.a: Full-length
Description:
Complex of recombinant full-length human p110α (GenBank Accession No. NM_006218.2) with N345K mutation and N-terminal His-Tag, and recombinant full length, human p85α (GenBank Accession No. NM_181523), no tag. Co-expressed in Sf9 cells in a Baculovirus expression system. p110α (N345K) MW=111 kDa, p85α MW=86 kDa.
Formulation:
50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Storage / Stability:
At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Samuels, Y., et al. Science 304: 554 (2008)

2. Graupera, M., et al., Nature 453: 662-666 (2008)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase
Product Type:
Recombinant Protein
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Product Datasheets

40646 Lot 161216.pdf