CSK (human, recombinant, N-terminal GST tag)
Catalog #: 40410
Size: 10 µg
Description: Human CSK (GenBank Accession No. NM_004383), full length with N-terminal
GST tag, MW= 80 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Source: Baculovirus infected Sf9 cells
Fomulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, 10
mM reduced glutathione, and 3 mM DTT.
Stability: >6 months at –80°C
Purity: >90% by SDS-PAGE.
Specific Activity: 100 U/mg. One unit is defined as the amount of enzyme that will transfer 1
nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES,
pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30
ug/ml Poly (Glu:Tyr)4:1 substrate, and 1 ug/ml recombinant CSK.
Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity