BRK
Human, recombinant, N-terminal GST tag

Catalog #: 40403
Lot #: 110418

Size: 10 µg

Description: Human BRK, also known
as PTK6 (GenBank Accession No.
NM_005975), full length, with N-terminal
GST tag, MW ~80 kDa, expressed in Sf9
insect cells via a baculovirus expression
system.

Specific Activity: 136 pmol/min/μg
Assay Buffer: 5 mM MOPS, 2.5 mM β-
glycerophosphate, 5 mM MgCl2, 1 mM
EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2
mM ATP. Incubate BRK with 0.5 mg/ml
Poly (Glu:Tyr, 4:1) peptide substrate and
[33P]-ATP at 30°C for 15 minutes, then
spot reaction on phosphocellulose paper,
fix in 1% phosphoric acid, and assay with
a scintillation counter.

Application:
Useful for the study of enzyme kinetics,
screening inhibitors, and selectivity
profiling.

Formulated in: 50 mM Tris-HCl, pH 7.5,
150 mM NaCl, 0.1 mM EDTA, 0.1 mM EGTA,
0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Stability: >12 months at -80°C

References:
1. Mitchell, P.J., et al. Oncogene 9(8): 2383-
2390 (1994).
2. Mitchell, P.J., et al. Oncogene 15(12): 1497-
1502 (1997).

PRODUCT DATA SHEETS
Lot 110418
Lot 110419

Data shown is lot-specific. For specific information on other lots, please contact us.