CSF1R / SRE – Reporter HEK293 Recombinant Cell Line

Catalog #
79380
$10,855 *
Size: 2 Vials
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The CSF1R / SRE – Reporter HEK293 Recombinant Cell Line has been transfected with full-length human CSF1R cDNA (NP_005202) under a CMV promoter for high constitutive expression. The SRE–luciferase reporter is also stably integrated into the genome. The firefly luciferase gene is controlled by 4 copies of the Serum Response Element upstream of a minimal promoter. Upon ligand binding, active CSF1R will initiate the MAPK/ERK signaling pathway, leading to expression of the SRE-controlled luciferase reporter.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of CSF1R without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
CSF1R, CSFR, CD115, M-CSF-R
Product Info
Storage and Usage
Citations
Host Cell Line
HEK293
Species
Human
Supplied As
Each vial contains ~2 X 106 cells in 1 ml of 90% FBS, 10% DMSO
Materials Required But Not Supplied

Materials Required for Cell Culture

Name Ordering Information
Thaw Medium 1 BPS Bioscience #60187
Growth Medium 1M BPS Bioscience #79723


Materials Required for Cellular Assay

Name Ordering Information
Thaw Medium 1 BPS Bioscience #60187
Growth Medium 1M BPS Bioscience #79723
Assay Medium 1B BPS Bioscience #79617
Recombinant human M-CSF or Recombinant human IL-34 BioLegend #574802 or R&D Systems #5265-IL/CF
Pexidartinib/PLX3397 SelleckChem #S7818
96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate  
ONE-Step™ Luciferase Assay System BPS Bioscience #60690
Luminometer  
Formulation

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin 

Growth Medium 1M (BPS Bioscience #79723): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin , 400 µg/ml of Geneticin and 0.5 µg/ml Puromycin Dihydrochloride 

Assay Medium 1B (BPS Bioscience #79617): MEM medium supplemented with 0.5% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

UniProt #
P07333
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Colony Stimulating Factor 1 Receptor (CSF1R, CSFR, CD115, M-CSF-R) is a single-pass tyrosine kinase transmembrane receptor which is part of the type III protein tyrosine kinase receptor family. CSF1R is activated by either of two cytokines, CSF1 (MCSF; CSF-1) and IL-34 (IL34), causing homodimerization and activation of downstream kinase activity. CSF1R is expressed on the surface of monocytes and macrophages, and its activation controls the growth, function, and differentiation of macrophages. This interaction is used by numerous cancer types, such as diffuse-type tenosynovial giant cell tumors (dt-GCT), to evade the immune system. By overexpressing the cytokine CSF1, these cells drive the development and survival of Tumor-Associated Macrophages (TAMs), which in turn suppress the local immune response to the cancer.

Activation of CSF1R by its ligand initiates a vast array of intracellular activity, including activation of the MAPK/ERK signaling pathway. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.

References

1. Cannarile, Michael A., et al. 2017. “Colony-Stimulating Factor 1 Receptor (CSF1R) Inhibitors in Cancer Therapy.” Journal for Immunotherapy of Cancer, 5(1),(53), doi:10.1186/s40425-017-0257-y.
2. Liu, Yang, and Xuetao Cao. 2014. “The Origin and Function of Tumor-Associated Macrophages.” Cellular and Molecular Immunology, 12(1), 1–4., doi:10.1038/cmi.2014.83.
3. Luo, Jian, et al. 2013. “Colony-Stimulating Factor 1 Receptor (CSF1R) Signaling in Injured Neurons Facilitates Protection and Survival.” The Journal of Experimental Medicine, 210(1), 157–172., doi:10.1084/jem.20120412.
4. Yao, G.-Q., et al. 2005. “CSF-1 Induces Fos Gene Transcription and Activates the Transcription Factor Elk-1 in Mature Osteoclasts.” Calcified Tissue International, 76(5),  371–378., doi:10.1007/s00223-004-0099-8.