TrkA Assay Kit

Catalog #: 79548
Size: 96 reactions
$495
Description:
The tropomyosin receptor kinases (TRK) are a family of tyrosine receptor kinases, which include TrkA, TrkB and TrkC. TrkA is activated by binding to nerve growth factor (NGF), resulting in activation of cell proliferation through the RAS/MAPK/ERK and PLCγ/PI3K pathways. Constitutive activation of TrkA is associated with colorectal cancer, suggesting TrkA inhibitors may have potential therapeutic value. The TrkA Assay Kit is designed to measure TrkA activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The TrkA Assay Kit comes in a convenient 96-well format, with enough purified recombinant TrkA enzyme, TrkA substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Provided:

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

Format:

Storage / Stability:
Up to 6 months when stored as recommended
Application(s):
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Scientific Category:
Kinase
Product Type:
Assay Kit
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Product Datasheets

79548.pdf