JAK2 (Janus Kinase 2) Assay Kit
Janus kinase (JAK) is a family of intracellular, nonreceptor tyrosine kinases that play an important role in cytokine-mediated signaling. This family includes four protein kinases; JAK1, JAK2, JAK3 and Tyk2. Various studies have suggested that regulation of JAK activity should be a promising approach to combat numerous diseases such as immune deficiencies and cancers. The JAK2 (Janus Kinase 2) Assay Kit is designed to measure JAK2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
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This product has been cited 4 times.
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
Catalog Number |
Reagent |
Amount |
Storage |
|
40450 | JAK2 | 5 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
79334 | 5x Kinase assay buffer 1 | 1.5 ml | -20°C | |
79686 | ATP (500 μM) | 100 µl | -20°C | |
40217 | PTK substrate Poly(Glu:Tyr 4:1) (10 mg/ml) |
100 µl | -20°C | |
79696 | 96-well plate, white | 1 | Room Temp. |
Alicea, N.L. et al., Current Drug Targets 12(4):546-555 (2011)