JAK2 (Janus Kinase 2) Assay Kit

Catalog #
79520
$560 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

Janus kinase (JAK) is a family of intracellular, nonreceptor tyrosine kinases that play an important role in cytokine-mediated signaling. This family includes four protein kinases; JAK1, JAK2, JAK3 and Tyk2. Various studies have suggested that regulation of JAK activity should be a promising approach to combat numerous diseases such as immune deficiencies and cancers. The JAK2 (Janus Kinase 2) Assay Kit is designed to measure JAK2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against JAK2 (Janus Kinase 2)? Check out our Kinase Screening Services.

This product has been cited 4 times.

Synonyms
Tyrosine-protein kinase JAK2, JAK-2
Product Info
Storage and Usage
Citations4
Assay Kit Format
Luminescent
Supplied As
The JAK2 (Janus Kinase 2) Assay Kit comes in a convenient 96-well format, with enough purified recombinant JAK2 enzyme, JAK2 substrate peptide (Poly(Glu;Tyr, 4:1)), ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format
Catalog
Number 

Reagent

Amount

Storage
40450 JAK2 5 µg -80°C

Avoid multiple
freeze/
thaw
cycles!
79334 5x Kinase assay buffer 1 1.5 ml -20°C
79686 ATP (500 μM) 100 µl -20°C
40217 PTK substrate Poly(Glu:Tyr 4:1)
(10 mg/ml)
100 µl -20°C
79696 96-well plate, white 1 Room Temp.
UniProt #
O60674
References

Alicea, N.L. et al., Current Drug Targets 12(4):546-555 (2011)