GSK3β Assay Kit

Catalog #
79700
$590 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The GSK3β Assay Kit is designed to measure GSK3β activity for screening and profiling applications using Kinase-Glo® (Promega) as a detection reagent. The GSK3β Assay Kit comes in a convenient 96-well format, with enough purified recombinant GSK3β enzyme, GSK3β substrate (GSK substrate peptide), ATP, and kinase assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against GSK3ß? Check out our Kinase Screening Services.

This product has been cited 6 times.

Synonyms
Glycogen Synthase Kinase 3 Beta, GSK-3 Beta, Serine/Threonine-Protein Kinase GSK3B
Product Info
Storage and Usage
Citations6
Assay Kit Format
Luminescent
Materials Required But Not Supplied

Kinase-Glo® Max Assay (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format
Catalog
Number
Reagent Amount Storage
40007 GSK3β* 1.5 µg -80°C

Avoid multiple
freeze/thaw
cycles!

79334 5x Kinase assay buffer 1.5 ml -20°C
79686 ATP (500 μM) 100 µl -20°C
79697

GSK substrate peptide (1 mg/ml)

500 µl -20°C
79696 96-well plate, white 1 Room Temp.

 

 

 

 

 

 

 

 

 

 

 

*The initial concentration of GSK3ß is lot-specific and will be indicated on the tube containing the enzyme.

UniProt #
P49841
Background
GSK3 (Glycogen synthase kinase) is a serine/threonine kinase which initially was found to phosphorylate and inactivate glycogen synthase in glycogen biosynthesis. It is an important regulator enzyme in many disease pathogeneses including cancer, immune disorders, metabolic disorders and neurological disorders like Alzheimer’s disease. In fact, GSK3β, one of the isoforms of GSK3, was identified as a key regulator in tau-driven Alzheimer’s disease.