GSK3β Assay Kit
The GSK3β Assay Kit is designed to measure GSK3β activity for screening and profiling applications using Kinase-Glo® (Promega) as a detection reagent. The GSK3β Assay Kit comes in a convenient 96-well format, with enough purified recombinant GSK3β enzyme, GSK3β substrate (GSK substrate peptide), ATP, and kinase assay buffer for 100 enzyme reactions.
Need us to run inhibitor screens or profile your compounds against GSK3ß? Check out our Kinase Screening Services.
This product has been cited 6 times.
Kinase-Glo® Max Assay (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
Catalog Number |
Reagent | Amount | Storage | |
40007 | GSK3β* | 1.5 µg | -80°C |
Avoid multiple |
79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
79686 | ATP (500 μM) | 100 µl | -20°C | |
79697 |
GSK substrate peptide (1 mg/ml) |
500 µl | -20°C | |
79696 | 96-well plate, white | 1 | Room Temp. |
*The initial concentration of GSK3ß is lot-specific and will be indicated on the tube containing the enzyme.
McCubrey JA., et al. Oncotarget 5(10): 2881-2911 (2014)
Application Reference(s):
1. Multitarget Approach to Drug Candidates against Alzheimer\'s Disease Related to AChE, SERT, BACE1 and GSK3β Protein Targets (2020)
2. Kynurenine 3-monooxygenase upregulates pluripotent genes through β-catenin and promotes triple-negative breast cancer progression (2020)