GSK3α Assay Kit

Catalog #

79698

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The GSK3α Assay Kit is designed to measure GSK3α activity for screening and profiling applications using Kinase-Glo® (Promega) as a detection reagent.

Need us to run inhibitor screens or profile your compounds against GSK3α? Check out our Kinase Screening Services.

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Synonyms

Glycogen Synthase Kinase 3 Alpha, GSK-3 Alpha, Serine/Threonine-Protein Kinase GSK3A

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

Kinase-Glo® Max Assay (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Components	Amount	Storage
40006	GSK3α*	1.5 µg	-80°C	Avoid freeze/ thaw cycles!
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
79697	1 mg/ml GSK substrate peptide	500 µl	-20°C
79696	96-well plate, white	1	Room Temp.

*The concentration of GSK3α is lot-specific and will be indicated on the tube containing the enzyme.

UniProt #

P49840

Background

GSK3 (Glycogen synthase kinase) is a serine/threonine kinase which initially was found to phosphorylate and inactivate glycogen synthase in glycogen biosynthesis. It is an important regulator enzyme in many disease pathogeneses including cancer, immune disorders, metabolic disorders and neurological disorders like Alzheimer’s disease. Recently, GSK3α, one of the isoforms of GSK3, was identified as a key target in acute myeloid leukemia (AML).

References

McCubrey JA., et al. Oncotarget 5(10): 2881-2911 (2014)

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay protocol.

Shipping Temperature

-80°C

Contraindications

Keep final DMSO concentration below 1%

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only