FAK Kinase Assay Kit

Catalog #: 40722
Size: 96 reactions
$495
Background:
FAK (Focal Adhesion Kinase; PTK2) is a protein kinase involving cell adhesion and migration. FAK plays an important role in many cancers including pancreatic tumors. In addition, recent studies showed FAK inhibitor enables an anti-tumor immune response, resulting in tumor destruction in a mouse model.
Description:
The FAK Kinase Assay Kit is designed to measure FAK kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Synonym(s):
FAK, focal adhesion kinase, PTK2
Supplied As:
FAK Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant FAK enzyme, FAK substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol.
Application(s):
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Jiang, H. et al., Nature Medicine 22:851-860 (2016)
Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Kinase-Glo MAX (Promega, #V6071)
Dithiothreitol (DTT, 1 M; optional) 
Microplate reader capable of reading luminescence 
Adjustable micropipettor and sterile tips
30°C incubator

Scientific Category:
Kinase
Product Type:
Assay Kit
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Product Datasheets

40722.pdf