FAK Kinase Assay Kit

Catalog #

40722

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The FAK Kinase Assay Kit is designed to measure FAK kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against FAK? Check out our Kinase Screening Services.

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Synonyms

FAK, focal adhesion kinase, PTK2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The FAK Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant FAK enzyme, FAK substrate, ATP and Kinase Buffer 1 for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega, #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40420	FAK	10 µg	-80°C	Avoid multiple freeze/ thaw cycles!
79334	5x Kinase Buffer 1	1.5ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
40217	PTK substrate Poly (Glu: Tyr 4:1) (10mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

Q05397

Background

FAK (Focal Adhesion Kinase; PTK2) is a protein kinase involving cell adhesion and migration. FAK plays an important role in many cancers including pancreatic tumors. In addition, recent studies showed FAK inhibitor enables an anti-tumor immune response, resulting in tumor destruction in a mouse model.

References

Jiang, H. et al., Nature Medicine 22:851-860 (2016)