EGFR(T790M) Kinase Assay Kit

Catalog #
40323
$535 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cellsurface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. EGFR(T790M) mutation is found as a second-site mutation in ~50% of EGFR-mutated lung tumors. The mutant was shown to be resistant to most tyrosine kinase inhibitor medicines including Erlotinib and Gefitinib. The EGFR(T790M) Kinase Assay Kit is designed to measure EGFR (T790M) Kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

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Synonyms
EGFR, ErbB-1, HER1
Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Supplied As
The EGFR (T790M) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR(T790M) enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format
Catalog
Number
Reagent Amount Storage
40188 EGFR (T790M)  2 µg -80°C  Avoid
multiple
freeze/
thaw
cycles!
79334 5x Kinase Buffer 1.5 ml -20°C 
79686 ATP (500 µM) 100 µl  -20°C 
40217 PTK substrate Poly (Glu:Tyr 4:1) 
(10 mg/ml)
100 µl -20°C 
79696 96-well plate, white  1 Room Temp.
UniProt #
P00533
References

1. Nakamura, J.L. Expert Opin. Ther. Targets 11(4):463-472 (2007)

2. Suda, K., et al. J Thorac Oncol. Jan;4(1):1-4 (2009).