EGFR(T790M) Kinase Assay Kit

Catalog #: 40323
Size: 96 reactions
$495
Description:
The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. EGFR(T790M) mutation is found as a second-site mutation in ~50% of EGFR-mutated lung tumors. The mutant was shown to be resistant to most tyrosine kinase inhibitor medicines including Erlotinib and Gefitinib. The EGFR(T790M) Kinase Assay Kit is designed to measure EGFR (T790M) Kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The EGFR (T790M) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR(T790M) enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.
UniProt
P00533
Synonym(s):
EGFR, ErbB-1, HER1
Supplied As:
The EGFR (T790M) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR(T790M) enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

1. Nakamura, J.L. Expert Opin. Ther. Targets 11(4):463-472 (2007)

2. Suda, K., et al. J Thorac Oncol. Jan;4(1):1-4 (2009).

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Kinase
Product Type:
Assay Kit
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Product Datasheets

40323.pdf