CDK7 Assay Kit

Catalog #

79603

$535 *

Size: 96 Reactions

Qty

Bulk/Custom

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Description

Cyclin-dependent kinase 7 (CDK7) assembled with Cyclin H and MAT1 comprises an important sub-component of the transcription factor TFIIH. It is known to have dual roles, which include transcription regulation via phosphorylating the C-terminal domain (CTD) of RNA polymerase II as well as controlling cell cycle progression as a CDK activating kinase (CAK). The CDK7 Assay Kit is designed to measure CDK7/Cyclin H/MAT1 activity for screening and profiling applications, using ADP-Glo® as a detection reagent.

Need us to run inhibitor screens or profile your compounds against CDK7? Check out our Kinase Screening Services or DNA Replication and Repair Screening Services.

This product has been cited 5 times.

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Synonyms

Cyclin-dependent kinase 7, cyclin dependent kinase 7, CDK7, CDK-7

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Product Data Gallery

Product Info

Storage and Usage

Citations5

Assay Kit Format

Luminescent

Supplied As

The CDK7 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK7/Cyclin H/MAT1 enzyme, CDK substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Component	Amount	Storage
40098	CDK7/Cyclin H/MAT1	10 µg	-80°C	*Avoid* *freeze/* *thaw* *cycles!*
79334	5x Kinase assy buffer 1	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
79604	CDK substrate peptide 2 (1 mg/ml)	125 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

Q24216

References

Uzma A. et al., Nature Review Drug Discovery 14:130-146 (2015)

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only