CDK2 Assay Kit

Catalog #

79599

$535 *

Size: 96 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. CDKs are active only when bound to their regulator proteins, cyclins. CDK activity is tightly controlled for successful cell division. Since abnormal cell division represents cancer pathology, controlling CDK activity has been shown as a promising therapeutic strategy. In particular, CDK2 plays an important role in DNA replication. The CDK2 Assay Kit is designed to measure CDK2/CyclinA2 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent. The CDK2 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK2/CyclinA2 enzyme, CDK substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.

This product has been cited 13 times.

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Synonyms

cyclin-dependent kinase 2, CDKN2, cyclin dependent kinase 2, CDK-2

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Product Data Gallery

Product Info

Storage and Usage

Citations13

Assay Kit Format

Luminescent

Supplied As

The CDK2 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK2/CyclinA2 enzyme, CDK substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Reagent	Amount	Storage
41101	CDK2/CyclinA2	5 µg	-80°C	Avoid freeze/ thaw cycles!
79334	5x Kinase assay buffer 1	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
79598	10x CDK substrate peptide 1	500 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P24941 (CDK2), P20248 (CyclinA2)

References

Uzma A. et. al., Nature Review Drug Discovery 14:130-146 (2015)

Storage/Stability

Stable at least 6 months from date of receipt. when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only