AXL Kinase Assay Kit

Catalog #

79711

$535 *

Size: 96 reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The AXL Kinase Assay Kit is designed to measure AXL kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent. The AXL Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant AXL enzyme, AXL substrate (IRS1-tide), ATP and kinase assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against AXL? Check out our Kinase Screening Services.

●

Synonyms

AZF, AZFA, SP3, AZF1, ARK, JTK11, Tyro7, UFO, AXL receptor tyrosine kinase

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The AXL Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant AXL enzyme, AXL substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40180	AXL*	10 µg	-80°C	Avoid multiple freeze/thaw cycles!
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500µM)	100 µl	-20°C
79519	10x IRS1-tide (1mg/ml)	500 µl	-20°C
79696	96-well plate, white	1	Room Temp.

*The concentration of AXL is lot-specific and will be indicated on the tube containing the enzyme.

UniProt #

P30530

Background

AXL (TYRO7) is a receptor tyrosine kinase that is overexpressed in various forms of cancer, including breast, prostate, colorectal, and non-small cell cancers. AXL overexpression has been shown to encourage tumor progression and metastasis and to decrease the anti-tumor immune response, making AXL an attractive candidate for anticancer therapies.

References

1. Wu, X., Liu, X., Koul, S., Lee, C.Y., Zhang, Z., Halmos, B. AXL kinase as a novel target for cancer therapy. Oncotarget. 2014; 5(20):9546-63.

2. Rankin, E.B., Giaccia, A.J. The Receptor Tyrosine Kinase AXL in Cancer Progression. Cancers (Basel). 2016; 8(11):103.

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only