ABL (T315I) Assay Kit

Catalog #
79576
$535 *
Size: 96 reactions
Qty
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Description

The Abelson (ABL) family of nonreceptor tyrosine kinases is critical to the progression and treatment of chronic myeloid leukemia (CML). The single-point mutation T315I has been linked to drug resistance and poor prognosis, making it an important drug target. The ABL (T315I) Assay Kit is designed to measure ABL activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The ABL (T315I) Assay Kit comes in a convenient 96-well format, with enough purified recombinant ABL enzyme, ABLtide peptide, ATP, and kinase assay buffer for 100 enzyme reactions.

Synonyms
ABL Kit, ABL assay, T315I kit, Abelson murine leukemia
Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

Format

 

Catalog
Number
Reagent Amount Storage
40415 ABL (T315I)* >1 µg -80°C

Avoid multiple
freeze/thaw
cycles!

79334 5x Kinase assay buffer 1.5 ml -20°C
79686 ATP (500 μM) 100 µl -20°C
79577

ABLtide (1 mg/ml)

500 µl -20°C
79696 96-well plate, white 1 Room Temp.
UniProt #
P00519-1
References

1. Greuber, E. K., Smith-Pearson, P., Wang, J., & Pendergast, A. M. (2013). Role of ABL Family Kinases in Cancer: from Leukemia to Solid Tumors. Nature Reviews. Cancer, 13(8), 559–571.

2. Ganguly SS, Plattner R. (2012). Activation of Abl Family Kinases in Solid Tumors. Genes & Cancer 3(5-6):414-425.