ALK2 Kinase Assay Kit

Catalog #: 79605
Size: 96 Reactions
$495
Background:
ALK2, also known as activin receptor-like kinase 2 (ACVRLK2) is a receptor from the TGF-β family of transmembrane serine/threonine kinases that plays an important role in the bone morphogenic protein (BMP) pathway. Mutation of ALK2 is seen to play a role in cancer and fibrodysplasia ossificans progressiva (FOP).
Description:
The ALK2 Kinase Assay Kit is designed to measure ALK2 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
Materials Required But Not Provided:
  • ADP-Glo® Kinase Assay (Promega #V6930) 
  • Dithiothreitol (DTT, 1 M; optional) 
  • Microplate reader capable of reading luminescence 
  • Adjustable micropipettor and sterile tips 
  • 30°C incubator
Synonym(s):
Activin receptor-like kinase 2 (ACVRLK2)
Supplied As:
The ALK2 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant ALK2 enzyme, ALK2 substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed/ Kit components require different storage conditions.Be sure to store each component at the proper temperature upon arrival.
Application(s):
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

1. Cunha, Sara I., and Kristian Pietras. “ALK2 as an Emerging Target for Antiangiogenic Therapy of Cancer.” Blood 117(26): 6999–7006 (2011).

2. Minsung Kim, et al. Identification of novel ALK2 inhibitors and their effect on cancer cells, Biochemical and Biophysical Research Communications, 492(1):121-127 (2017).

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:
Kinase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

79605.pdf