14-3-3 epsilon Protein, His-tag (Human)

Catalog #: 79025
Size: 20 μg
Species: Human
Host Species: Sf9 insect cells using baculovirus
MW: 30 kDa
Genbank #: NM_006761
Tag(s): N-terminal His tag
a.a: YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465
14-3-3 epsilon also known as tyrosine 3-mono-oxygenase/tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. 14-3-3 epsilon has been reported to be involved in heat shock transcription factor 1 (HSF1) and extracellular signal regulated protein kinase (ERK) pathways (1). 14-3-3 epsilon interaction with the deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Association of 14-3-3 epsilon, HSF1 with ERK during heat shock may modulate the amplitude of the response and control the termination of HSP expression on resumption of growth conditions (2).
Human 14-3-3 epsilon Protein, GenBank Accession No. NM_006761, full length, with N-terminal His tag, expressed in Sf9 insect cells using baculovirus. MW = 30 kDa.
YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
Storage / Stability:
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western blot
1. Wang, X. et al: Interactions between extracellular signal-regulated protein kinase 1, 14-3-3epsilon, and heat shock factor 1 during stress. J. Biol. Chem. 2004; 279(47):49460-9.

2. Mizuno, E. et al: 14-3-3-dependent inhibition of the deubiquitinating activity of UBPY and its cancellation in the M phase. Exp Cell Res. 2007; 313(16):3624-34.

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