AP-1 Reporter – HEK293 Recombinant Cell Line (JNK signaling pathway)

Catalog #
60405
$2,445 *
Size: 2 vials
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun.  c-Jun then binds to the activator protein-1 (AP1) response element and induces AP1 transcription.

The AP1 Reporter – HEK293 cell line contains a firefly luciferase gene under the control of AP1-responsive elements that are stably integrated into HEK293 cells. This cell line is validated for its response to stimulation by Phorbol 12-Myristate 13-Acetate (PMA) and to treatment with inhibitors of the JNK signaling pathway.

Interested in screening and profiling inhibitors or activators of SAPK/JNK pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 2 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
JNK reporter, AP-1 cell line, activator protein-1 cell line, activator protein-1, JNK-1, JNK-2
Product Info
Storage and Usage
Citations2
Host Cell Line
HEK293 cells
Supplied As
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Materials Required But Not Supplied

Materials Required but Not Supplied for Cell Culture

Materials Required but Not Supplied for Cellular Assay

Biological Activity
This cell line is validated for its response to stimulation by Phorbol 12-Myristate 13-Acetate (PMA) and to treatment with inhibitors of the JNK signaling pathway.
UniProt #
P45983, P45984, P53779
Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.