JMJD3 (KDM6B), FLAG-tag

Catalog #: 50115
Size: 20 µg
$460
Species: Human
Host Species: Sf9 insect cells
MW: 71 kDa
Genbank #: NM_001080424
Tag(s): C-terminal FLAG-tag
a.a: 1043–end
Description:
Human JMJD3 (KDM6B) histone demethylase (a.a. 1043-end), Genbank Accession No. NM_001080424, with C-terminal FLAG-tag, MW=71 kDa, expressed in Sf9 cells via a baculovirus expression system.
UniProt
O15054
Synonym(s):
JMJD3, KDM6B
Specific Activity:
1.1 pmole/min/μg
Assay Conditions:
10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD3 (100 – 500 ng) added to the wells. Add antibody against demethylated K27 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl and 20% Glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Y. Xiang, et al., Cell Research (2007) 17:850–8572.
2. S. Hong, et al. Proc. Natl. Acad. Sci. USA (2007) 104(47):18439-44.
3. Swigut T, Wysocka J. Cell (2007) 131(1):29-3.

Application References:
1. A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
2. Identification of small molecule inhibitors of Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase by a sensitive high throughput screen (2013)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
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Product Datasheets

50115_Lot 161202.pdf