JMJD2E (KDM4DL) Homogeneous Assay Kit

Catalog #
50417
$1,875 *
Size: 384 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The JMJD2E Homogeneous Assay Kit is designed to measure activity of JMJD2E for screening and profiling applications, using AlphaLISA® technology. The key to the JMJD2E Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2E enzyme is incubated with the biotinylated substrate for two hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alphacounts.

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Synonyms
JMJD2E, KDM4DL, lysine (K)-specific demethylase 4D-like
Product Info
Storage and Usage
Citations
Assay Kit Format
AlphaLISA®
Supplied As
Kit comes in a convenient AlphaLISA® format, with a 384-well plate, biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JMJD2E for 384 enzyme reactions.
Format
Catalog
Number

Component

Amount

Storage
50118 JMJD2E (KDM4DL) 60 μg -80°C

Avoid
freeze/
thaw
cycles!
52140E Primary antibody 5 20 μl -80°C
79841 Biotinylated histone H3 peptide substrate 500 rxns -80°C
52408 4x HDM Assay Buffer 3 3 ml -80°C
52031 4x Detection buffer 2 ml -20°C

 

UniProt #
B2RXH2
Background
JMJD2E, also known as KDM4DL (lysine (K)-specific demethylase 4D-like) is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3.
References
Whetstine, J.R. et al., Cell 2006; 125: 467.