JMJD2D (KDM4D), GST-tag

Catalog #: 50117
Size: 100 µg
$425
Species: Human
Host Species: Sf9 cells
MW: 67 kDa
Genbank #: NM_018039
Tag(s): N-terminal GST-tag
a.a: 2–354
Description:
Human JMJD2D (GenBank Accession No. NM_018039), amino acids 2–354, with N-terminal GST tag, MW=67 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q6B0I6
Synonym(s):
KDM4D
Specific Activity:
≥ 0.14 pmole/min/μg
Assay Conditions:
10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1 mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2D (5 – 25 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Formulation:
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol
Storage / Stability:
At least 6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Katoh, M., Int J Oncol. 2004 Jun; 24(6):1623-8.
2. Shin, S., et al., Biochem Biophys Res Commun. 2007 Feb 23; 353(4):973-7.

Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
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Product Datasheets

50117_Lot_150408-G2.pdf