JMJD2D (KDM4D), GST-tag Recombinant

Catalog #

50117

$440 *

Size: 100 µg

Qty

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Description

Human JMJD2D (GenBank Accession No. NM_018039), amino acids 2–354, with N-terminal GST tag, MW=67 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 1 time.

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Synonyms

KDM4D

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

JMJD2D (KDM4D) inhibition curve using an AlphaLISA assay (#79838). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 cells

Purity

≥90%

Formulation

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol

67 kDa

Amino Acids

2–354

Specific Activity

≥ 0.14 pmole/min/μg

Genbank #

NM_018039

UniProt #

Q6B0I6

Tag(s)

N-terminal GST-tag

References

1. Katoh, M., Int J Oncol. 2004 Jun; 24(6):1623-8.
2. Shin, S., et al., Biochem Biophys Res Commun. 2007 Feb 23; 353(4):973-7.

Storage/Stability

At least 6 months at –80°C

Assay Conditions

10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1 mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2D (5 – 25 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Demethylase

Regulatory Status

Research Use Only