JMJD2C, GST-Tag

Catalog #: 50105
Size: 100 µg
$450
Species: Human
Host Species: Sf9 insect cells
MW: 70 kDa
Genbank #: BC143571
Tag(s): N-terminal GST-tag
a.a: 2–372
Description:
Human JMJD2C (GenBank Accession No. BC143571) (amino-acids 2-372) with N-terminal GST tag, MW=70 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q9H3R0
Synonym(s):
KDM4C, GASC1
Specific Activity:
0.26 pmole/min/μg
Assay Conditions:
10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2C (2 – 10ng) added to the wells. Add antibody against dimethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Formulation:
40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl and 20% glycerol.
Format:
Aqueous buffer solution.
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Vinatzer, U., et al., Clin Cancer Res. 2008 Oct 15;14(20):6426-31.
2. Pollard, P.J., et al., Biochem J. 2008 Dec 15;416(3):387-94.

Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
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Product Datasheets

50105.pdf