JMJD2C, GST-Tag Recombinant

Catalog #

50105

$465 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human JMJD2C (GenBank Accession No. BC143571) (amino-acids 2-372) with N-terminal GST tag, MW=70 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 2 times.

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Synonyms

KDM4C, GASC1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

JMJD2C (KDM4C) inhibition curve using an AlphaLISA assay (#50105). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl and 20% glycerol.

70 kDa

Amino Acids

2–372

Specific Activity

0.26 pmole/min/μg

Genbank #

BC143571

UniProt #

Q9H3R0

Tag(s)

N-terminal GST-tag

References

1. Vinatzer, U., et al., Clin Cancer Res. 2008 Oct 15;14(20):6426-31.
2. Pollard, P.J., et al., Biochem J. 2008 Dec 15;416(3):387-94.

Storage/Stability

>6 months at –80°C.

Assay Conditions

10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2C (2 – 10ng) added to the wells. Add antibody against dimethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Demethylase

Regulatory Status

Research Use Only