JMJD2B (KDM4B), GST-tag

Catalog #: 50111
Size: 100 µg
$450
Species: Human
Host Species: Sf9 insect cells
MW: 83 kDa
Genbank #: NM_015015
Tag(s): N-terminal GST-tag
a.a: 2–500
Description:
Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), a.a. 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.
UniProt
O94953
Synonym(s):
JHDM3B, KDM4B
Specific Activity:
0.32 pmole/min/µg
Assay Conditions:
10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mm KCl, 10% glycerol, 18 mM glutathione, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Fodor, B.D., et al. (2006). Genes Dev. 20(12): 1557–1562.
2. Beyer S., et al. (2008). J. Biol. Chem. 283(52):36542-52.

Application Reference(s):
1. Targeting Histone Demethylases in MYC-Driven Neuroblastomas with Ciclopirox (2017)
2. A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
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Product Datasheets

50111 Lot 150604.pdf